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Porcine liver vascular bed in Biodur E20 corrosion casts

EBERLOVÁ, L., LIŠKA, V., MÍRKA, H., GREGOR, T., TONAR, Z., PÁLEK, R., SKÁLA, M., BRŮHA, J., VYČÍTAL, O., KALUSOVÁ, K., HAVIAR, S., KRÁLÍČKOVÁ, M., LAMETSCHWANDTNER, A. Porcine liver vascular bed in Biodur E20 corrosion casts. Folia Morphologica, 2016, roč. 75, č. 2, s. 154-161. ISSN: 0015-5659
Jazyk publikace: eng
Anglický název: Porcine liver vascular bed in Biodur E20 corrosion casts
Rok vydání: 2016
Autoři: MUDr. Lada Eberlová , MUDr. Václav Liška , MUDr. Hynek Mírka , Ing. Tomáš Gregor PhD. , Doc. MUDr. et Mgr. Zbyněk Tonar Ph.D. , Richard Pálek , Martin Skála , MUDr. Jan Brůha , MUDr. Ondřej Vyčítal , MUDr. Kristýna Kalusová , RNDr. Stanislav Haviar Ph.D. , prof. MUDr. Milena Králíčková , Univ.Prof. Alois Lametschwandtner
Abstrakt EN: Pigs are frequently used as animal models in experimental medicine. To identify processes of vascular development or regression, vascular elements must be recognised and quantified in a 3D arrangement. Vascular corrosion casts enable the creation of 3D replicas of vascular trees. The aim of our study was to identify suitable casting media and optimise the protocol for porcine liver vascular corrosion casting. Mercox II® (Ladd Research, Williston, Vermont, USA) and Biodur E20® Plus (Biodur Products, Heidelberg, Germany) were tested in 4 porcine livers. The resins (volume approximately 700 mL) were injected via the portal vein. Corrosion casts were examined by macro-CT, micro-CT and scanning electron microscopy. For hepatectomies, the operating protocol was optimised to avoid gas or blood clot embolisation. Only Biodur E20® Plus appeared to be suitable for high-volume vascular corrosion casting due to its optimal permeability, sufficient processing time and minimum fragility. Biodur E20® Plus is slightly elastic, radio-opaque and alcohol-resistant. These properties make this acrylic resin suitable for not only vascular research but also teaching purposes.
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